Fig 4. Assessing the orange long-distance RNA-RNA interaction.

(A) RNA secondary structures of PRTE and DRTE in PEMV1. (B) and (D) Compensatory mutations introduced in the sg mRNA to test the orange interaction. Nucleotide substitutions are shown in red. (C) and (E) In vitro translation analyses of the wt and mutant sg mRNAs shown in panels B and D, respectively. Averaged Rel. RT levels (±SE) collected from three independent trials are shown below each lane.