TABLE 3.
Hemolysin levels and hemolytic activities in tolC mutants
| Strain | TolC | Alpha-hemolysin
|
||
|---|---|---|---|---|
| Zonea | Levelb | Activityb | ||
| RAM660 | TolC-wt | +++ | 100 | 100 |
| RAM959 | TolC− | − | 10 ± 10 | 2 ± 1 |
| RAM961 | L42P | + | 69 ± 11 | 4 ± 1 |
| RAM968 | T140A | + | 88 ± 2 | 10 ± 4 |
| RAM966 | G147D | ++ | 95 ± 5 | 28 ± 2 |
| RAM962 | S257P | ++c | 100 ± 14 | 9 ± 6 |
| RAM963 | A343T | + | 100 ± 8 | 46 ± 10 |
| RAM965 | A360T | ++ | 93 ± 2 | 51 ± 1 |
| RAM964 | A360V | ++ | 100 ± 1 | 47 ± 4 |
| RAM967 | T434M | ++ | 99 ± 1 | 49 ± 13 |
a
Qualitative measurements of hemolytic (halo) zones on blood agar plates are displayed.
b
Hemolysin activities and levels relative to the TolC+ strain. Averages from three independent experiments are shown.
c
Opaque hemolytic zone.