Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Trp63CreERT2 | Lee et al., 2014b; Lee et al., 2014a PMID:25210499 | Dr. Jianming Xu (Baylor College of Medicine) | |
| Genetic reagent (Mus musculus) | Tg(KRT5-CreERT2) | Rock et al., 2009 PMID:19625615 | Dr. Brigid Hogan (Duke University); A transgenic mouse strain in which human KRT5 promoter drives CreERT2 | |
| Genetic reagent (Mus musculus) | Scgb1a1CreERT |
Rawlins et al., 2009 PMID:19497281 |
MGI:3849566 | Dr. Brigid Hogan (Duke University) |
| Genetic reagent (Mus musculus) | Pou2f3CreERT2 | McGinty et al., 2020 PMID:32160525 | MGI:6755141 | Dr. Jakob von Moltke (University of Washington) |
| Genetic reagent (Mus musculus) | Tg(Trpm5-GFP) |
Clapp et al., 2006 PMID:16573824 |
16573824 | Dr. Tod Clapp (Colorado State University) |
| Genetic reagent (Mus musculus) | Trpm5-/- |
Damak et al., 2006 PMID:16436689 |
Dr. Robert Margolskee (Mount Sinai) | |
| Genetic reagent (Mus musculus) | Pou2f3-/- |
Matsumoto et al., 2011 PMID:21572433 |
MGI:5140071 | Dr. Keiko Abe (The University of Tokyo) |
| Genetic reagent (Mus musculus) | Rbpjkloxp/loxp |
Han et al., 2002 PMID:12039915 |
MGI:3583755 | Dr. Tasuku Honjo (Kyoto University) |
| Genetic reagent (Mus musculus) | B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J |
Madisen et al., 2010 PMID:20023653 |
MGI: 4436847 | Jackson Laboratories (#007914, R26Ai14) |
| Antibody | Anti-Dclk1 (Rabbit polyclonal) |
Abcam | ab31704 | 1:200 |
| Antibody | Anti-CC10 (E-11) (Mouse monoclonal) |
Santa Cruz | sc-365992 | 1:200; Scgb1a1 |
| Antibody | Anti-SCGB1A1 (Rat monoclonal) |
R&D | MAB4218 | 1:500 |
| Antibody | Anti-Krt5 (Chicken polyclonal) |
BioLegend | 905901 | 1:500 |
| Antibody | Anti-Krt5 (Mouse monoclonal) |
Abcam | ab17130 | 1:500 |
| Antibody | Anti-Krt5 (Rabbit polyclonal) |
Abcam | ab53121 | 1:500 |
| Antibody | Anti-p63 (Rabbit polyclonal) |
Genetex | GTX102425 | 1:200 |
| Antibody | Anti-p63 (Mouse monoclonal) |
Abcam | ab735 | 1:200 |
| Antibody | Anti-Acetylated tubulin (Mouse monoclonal) |
Sigma | T7451 | 1:500 |
| Antibody | Anti-TTF1 (Rabbit monoclonal) |
Abcam | ab76013 | 1:500; Nkx2.1 |
| Antibody | Anti-FOXA2 (Mouse monoclonal) |
Abcam | ab60721 | 1:200 |
| Antibody | Anti-POU2F3 (Rabbit polyclonal) |
Sigma | HPA019652 | 1:200 |
| Antibody | Anti-ABCA3 (Rabbit polyclonal) |
Seven Hills | WRAB-70565 | 1:500 |
| Antibody | Anti-Hop (E-1) (Mouse monoclonal) |
Santa Cruz | sc-398703 | 1:100; Hopx |
| Antibody | Anti- Prosurfactant Protein C (Rabbit polyclonal) |
Abcam | ab90716 | 1:500; SftpC |
| Antibody | Anti-Pro-SP-C (Rabbit polyclonal) |
Seven Hills | WRAB-9337 | 1:1000 |
| Antibody | Anti-tdTomato (Goat polyclonal) |
Biorbyt | orb182397 | 1:1000 |
| Antibody | Anti-Pdpn (Syrian hamster monoclonal) |
DSHB | 8.1.1 c | 1:500; T1α |
| Antibody | Anti- Green Fluorescent Protein (Chicken polyclonal) |
Fisher (Aves Lab) | GFP1020 | 1:200; GFP |
| Antibody | Anti-Ki-67 (Rat monoclonal) |
Invitrogen | 14-5698-82 | 1:50 |
| Antibody | Alexa Fluor 488 Donkey Anti-Chicken (Donkey polyclonal) |
Jackson Immuno Research | 703-546-155 | 1:500 |
| Antibody | Alexa Fluor 488 Donkey Anti-Mouse (Donkey polyclonal) |
Jackson Immuno Research | 715-545-151 | 1:500 |
| Antibody | Cy5 AffiniPure Donkey Anti-Rat IgG (Donkey polyclonal) |
Jackson Immuno Research | 712-175-150 | 1:500 |
| Antibody | Cy3 AffiniPure Donkey Anti-Mouse IgM (Donkey polyclonal) |
Jackson Immuno Research | 715-165-140 | 1:500 |
| Antibody | Alexa Fluor 488 Donkey anti-Rat IgG (Donkey polyclonal) |
Invitrogen | A21208 | 1:500 |
| Antibody | Alexa Fluor 555 Donkey anti-Rabbit IgG (Donkey polyclonal) |
Invitrogen | A31572 | 1:500 |
| Antibody | Alexa Fluor 555 Donkey anti-Goat IgG (Donkey polyclonal) |
Invitrogen | A21432 | 1:500 |
| Antibody | Alexa Fluor 555 Donkey anti-Mouse IgG (Donkey polyclonal) |
Invitrogen | A31570 | 1:500 |
| Antibody | Alexa Fluor 647 Goat anti-Chicken IgG (Goat polyclonal) |
Invitrogen | A21449 | 1:500 |
| Chemical compound, drug | DBZ | Tocris | 4489 | |
| Chemical compound, drug | Wnt-C59 | Tocris | 5148 | |
| Chemical compound, drug | CHIR99021 | Tocris | 4423 | |
| Chemical compound, drug | IWR-1 | Tocris | 3532 | |
| Chemical compound, drug | Dorsomorphin | Sigma | P5499 | |
| Chemical compound, drug | Verteporfin | Sigma | SML0534 | |
| Chemical compound, drug | GDC-0449 | Selleckchem | S1082 | |
| Chemical compound, drug | Dexamethasone | Sigma | D2915 | |
| Chemical compound, drug | 3-Isobutyl-1-methylxanthine (IBMX) | Sigma | I5879 | |
| Peptide, recombinant protein | Recombinant Il-6 | Peprotech | 200–06 | |
| Peptide, recombinant protein | Recombinant Il-4 | Peprotech | 200–13 | |
| Peptide, recombinant protein | Recombinant Murine Il-13 | Peprotech | 210–13 | |
| Chemical compound, drug | 8-Bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP) | Sigma | B5386 | |
| Chemical compound, drug | Bleomycin | Fresenius Kabi | 63323013610 | |
| Chemical compound, drug | Naphthalene | Sigma-Aldrich | 84679 | |
| Commercial assay or kit | Small Airway Epithelial Cell Medium | Lonza | CC-3118 | |
| Commercial assay or kit | Small Airway Epithelial Cell Medium | Promocell medium | C-21170 | |
| Commercial assay or kit | Complete Pneumacult-ALI medium | StemCell Technology | 05001 | |
| Other | Antigen unmasking solution, Citric acid based | Vector Laboratories | H-3300 | Antigen retrieval buffer for immunostaining |
| Other | Normal donkey serum | Jackson Immuno Research | 017-000-121 | Blocking reagent for immunostaining |