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. 2022 Oct 11;11:e72560. doi: 10.7554/eLife.72560

Figure 4. JZL184 treatment restores altered cannabinoid type-1 receptor (CB1R) density and coupling to Gi/o proteins in complete deletion (CD) mice.

(a) Quantification and (b) representative images of CB1R immunodetection in the basolateral amygdala of WT and CD mice after 10 days of treatment with vehicle (VEH) or JZL184 (8 mg/kg) (WT VEH, n = 6; WT JZL184, n = 4; CD VEH, n = 5; CD JZL184, n = 4). Scale bar = 100 µm. Statistical significance was calculated by Newman–Keuls post hoc test following two-way analysis of variance (ANOVA). *p < 0.05 (genotype effect); #p < 0.05, ###p < 0.001 (treatment effect). (c) [35S]GTPγS binding evoked by WIN55,212–2 (10 µM) after 10 days of treatment with vehicle (VEH) or JZL184 (8 mg/kg) (WT VEH, n = 5–8; WT JZL184, n = 6–9; CD VEH, n = 5–6; CD JZL184, n = 5) expressed as percentage of stimulation over the basal binding. Statistical significance was calculated by Newman–Keuls post hoc test following two-way ANOVA. **p < 0.01, ***p < 0.001 (genotype effect); #p < 0.05, ##p < 0.01, ###p < 0.001 (treatment effect). (d) Representative images of WIN55,212–2-evoked [35S]GTPγS binding. [14C]-microscales used as standards in Ci/g t.e. Scale bar = 5 mm. Data are expressed as mean ± standard error of the mean (SEM).

Figure 4—source data 1. JZL184 treatment restores altered cannabinoid type-1 receptor (CB1R) density and coupling.

Figure 4.

Figure 4—figure supplement 1. JZL184 treatment downregulates cannabinoid type-1 receptor (CB1R) protein levels in the amygdala of complete deletion (CD) mice (CD VEH, n=7; CD JZL184, n=7).

Figure 4—figure supplement 1.

Statistical significance was calculated by Student’s t-test. # p<0.05 (treatment effect). Data are expressed as mean ± S.E.M.
Figure 4—figure supplement 1—source data 1. JZL184 treatment downregulates cannabinoid type-1 receptor (CB1R) protein levels in the amygdala of complete deletion (CD) mice.
Figure 4—figure supplement 1—source data 2. Original blots of cannabinoid type-1 receptor (CB1R) in the amygdala of complete deletion (CD) mice.
Figure 4—figure supplement 2. Quantification of total number of cells and neurons in basolateral amygdala.

Figure 4—figure supplement 2.

The number of cells and neurons is not altered neither in CD mice or JZL184 treatment condition in Basolateral amygdala. (a) Density of NeuN+ and (b) Dapi + cells in basolateral amygdala measured by immunofluorescence. (WT VEH, n=6; WT JZL184, n=4; CD VEH, n=5; CD JZL184, n=4). Statistical significance was calculated by Newman-Keuls post hoc test following two-way ANOVA. Data are expressed as mean ± S.E.M.
Figure 4—figure supplement 2—source data 1. Quantification of total number of cells and neurons in basolateral amygdala.