Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, male) | C57BL/6J | Charles Rivers, France | C57Bl/6J | Male |
| Genetic reagent (Mus musculus) | CD | University Pompeu Fabra | (C57BL/6J background, male) | |
| Chemical compound, drug | JZL184 | Abcam | ab141592 | |
| Chemical compound, drug | Dimethyl sulfoxide | Scharlau Chemie | SU01531000 | |
| Chemical compound, drug | Polyethylene glycol 400 | AppliChem | 142436.1611 | |
| Chemical compound, drug | Tween-80 | Sigma-Aldrich | P1754 | |
| Chemical compound, drug | 0.9%, NaCl physiological saline | Laboratorios Ern | Vitulia | |
| Chemical compound, drug | Ketamidor | Richter pharma | K1NAI027 | |
| Chemical compound, drug | Xylazine hydrochloride | Sigma-Aldrich | X1251 | |
| Biological sample (Equus asinus) | Normal donkey serum | Sigma-Aldrich | D9663-10ML | 3% in PBS with 0.3% Triton X-100 |
| Antibody | Anti-CB1R (rabbit polyclonal) |
Immunogenes | 1:1000 immunofluorescence |
|
| Antibody | Anti-CB1R (rabbit polyclonal) |
Frontier science | CB1-Rb-Af380-1 | 1:500 western blot |
| Antibody | Anti-NeuN (mouse monoclonal) |
Merck Millipore | MAB377 | 1:1000 |
| Antibody | Anti-rabbit IgG (H+L)-AlexaFluor-555 (donkey polyclonal) |
Thermo Fisher Scientific | A-31572 | 1:1000 |
| Antibody | Anti-mouse IgG (H+L)-AlexaFluor-488 (goat polyclonal) |
Jackson ImmunoResearch | 115-545-003 | 1:1000 |
| Commercial assay, kit | Immobilon Forte Western HRP Substrate | Merck Millipore | WBLUF0500 | |
| Commercial assay, kit | Nucleospin RNA isolation kit | Macherey-Nagel | 740955-250 | |
| Commercial assay, kit | SuperScript III enzyme | Invitrogen | 12574-026 | |
| Commercial assay, kit | SybrGreen master mix | Thermo Fisher | 4309155 | |
| Sequence-based reagent | Primers Cnr1 (CB1R) (forward) | Sigma-Aldrich | 5′-CCTGGGAAGTGTCATCTTTGT-3′ | |
| Sequence-based reagent | Primers Cnr1 (CB1R) (reverse) | Sigma-Aldrich | 5′-GGTAACCCCACCCAGTTTGA-3′ | |
| Sequence-based reagent | Primers Gapdh (GAPDH) (forward) | Sigma-Aldrich | 5′-TGTCGTGGAGTCTACTGGTGTCTT-3′ | |
| Sequence-based reagent | Primers Gapdh (GAPDH) (reverse) | Sigma-Aldrich | 5′-TGGCTCCACCCTTCAAGTG-3′ | |
| Sequence-based reagent | Primers Hprt1 (HPRT) (forward) | Sigma-Aldrich | 5′-AAGCTTGCTGGTGAAAAGGA-3′ | |
| Sequence-based reagent | Primers Hprt1 (HPRT) (reverse) | Sigma-Aldrich | 5′-TTGCGCTCATCTTAGGCTTT-3′ | |
| Chemical compound, drug | DAPI Fluoromount-G mounting media | Thermo Fisher Scientific | 00-4959-52 | |
| Software, algorithm | GraphPad Prism 7 | GraphPad Software, Inc | RRID:SCR_002798 | |
| Software, algorithm | STATISTICA 6.0 | StatSoft, USA | RRID:SCR_014213 | |
| Software, algorithm | Smart 3.0 videotracking software | Panlab | RRID:SCR_002852 | |
| Software, algorithm | ImageJ | National Institutes of Health, Bethesda, Maryland, USA | RRID:SCR_003070 | |
| Software, algorithm | The Quantity One software v4.6.3 | Bio-Rad | RRID:SCR_014280 | |
| Software, algorithm | Salmon | PMID:28263959 | RRID:SCR_017036 | v0.7.2 |
| Software, algorithm | R | https://www.R-project.org/ | RRID:SCR_001905 | v3.6.3 |
| Software, algorithm | tximport | PMID:26925227 | RRID:SCR_016752 | v1.2.0 |
| Software, algorithm | DESeq2 | PMID:25516281 | RRID:SCR_015687 | v1.26.0 |
| Software, algorithm | clusterProfiler | PMID:22455463 | RRID:SCR_016884 | v3.14.3 |
| Chemical compound, drug | SR141716A | Tocris | 158681-13-1 | |
| Chemical compound, drug | SR144528 | Tocris | 192703-06-3 | |
| Chemical compound, drug | CP55,940 | Tocris | 83002-04-4 | |
| Chemical compound, drug | WIN55,212–2 | Sigma-Aldrich | 131543-23-2 | |
| Chemical compound, drug | Tris–HCl | Sigma-Aldrich | 1185-53-1 | |
| Chemical compound, drug | BSA | Sigma-Aldrich | 9048-46-8 | |
| Chemical compound, drug | HEPES | Sigma-Aldrich | 7365-45-9 | |
| Chemical compound, drug | NaCl | Sigma-Aldrich | 7647-14-5 | |
| Chemical compound, drug | MgCl2 | Sigma-Aldrich | 7791-18-6 | |
| Chemical compound, drug | EGTA | Sigma-Aldrich | 13368-13-3 | |
| Chemical compound, drug | GDP | Sigma-Aldrich | 43139-22-6 | |
| Chemical compound, drug | DTT | Sigma-Aldrich | 3483-12-3 | |
| Chemical compound, drug | GTPγS | Sigma-Aldrich | 10220647001 | |
| Chemical compound, drug | [3H]CP55,940 | PerkinElmer | NET1051250UC | |
| Chemical compound, drug | [35S]GTPγS | PerkinElmer | NEG030H250UC | |
| Other | β-Radiation sensitive film | Sigma-Aldrich | F5263-50EA | See autoradiography methodology |