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. 2022 Sep 8;11:e78074. doi: 10.7554/eLife.78074

Figure 1. Epcam+ Trpm5-GFP+ cells are bona fide tuft cells in the lung post-influenza.

(A) Representative immunostaining of lung sections from Trpm5-GFP reporter mice at day 35 post-influenza. Nuclear stain (DAPI) in blue, Trpm5-GFP in green, and POU2F3 in red. (B) Experimental design outlining the bulk RNA sequencing (RNA-Seq) experiment. (C) Trpm5-GFP reporter expression in live lung epithelial (Epcam+) cells post-influenza via FACS. (D) Volcano plot and (E) heatmap comparing gene expression between Epcam+Trpm5-GFP+ (tuft cells) and Epcam+Trpm5-GFP- (non-tuft epithelial) cells from mice at day 43 post influenza.*=Representative genes that have been previously associated with tuft cells.

Figure 1.

Figure 1—figure supplement 1. Gene expression comparison of post-influenza A virus (IAV) lung tuft cells and tracheal cells (Nadjsombati et al., 2018) using bulk RNA sequencing data.

Figure 1—figure supplement 1.

(A) Volcano plot showing differentially expressed genes between post-IAV lung tuft cells and tracheal tuft cells. (B) Heatmap of expression levels of canonical tuft cell markers. (C) Heatmap comparing additional shared/core tuft cell markers as well as Tuft-1 and Tuft-2 markers, indicating a reduced Tuft-1 character among trachea tuft cells.