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. 2022 Sep 8;11:e78074. doi: 10.7554/eLife.78074

Figure 5. Tuft cells are not required for goblet cell differentiation after influenza.

Figure 5.

(A–I) Krt5 (green) and Agr2 (red) staining and quantification 25 days after influenza. Agr2+ area per Krt5+ area was not significantly different between lung sections of (C) control (n=8) and Pou2f3-/- (n=8), (F) control (n=4) and Trpm5-/- (n=6) and (I) control (n=3) and Il4ra-/- (n=3). (J–M) Krt5 (green) and Muc5b (red) staining 25 days after influenza demonstrates Muc5b staining in (J–K) control (n=4) and Pou2f3-/- (n=3) and (L–M) control (n=4) and Il4ra-/- (n=3) dysplastic alveolar regions and (N) qRT-PCR (quantitative RT-PCR) for relative mRNA levels for goblet cell markers in control (n=8) and Pou2f3-/- (n=4) lungs 51 days after influenza, expression normalized to Actb (left) and Krt5 (right). (A–H) Scale bar is 500 µm, (J–M) scale bar is 200 µm.

Figure 5—source data 1. Quantification of Agr2 immunostaining and expression of goblet cell transcripts.