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LPA activates Gα12/13–Rho–ROCK2 by upregulating LPA4 and LPA6. (a) Activation of the Gα12/13 pathway is detected by the serum response factor response element (SRF-RE) luciferase reporter gene. (b, c) The expression of ROCK1 and ROCK2 in spinal cord neurons were measured by western blotting. Asterisks indicate statistical significance (∗p < 0.05, ∗∗p < 0.01).
