Table 4.
Various samples of antitumor activity of H. digitata.
| Sample content | Concentration (μg/ml) | Inhibition in average (mean ± SEM) | Inhibition percentage (mean ± SEM) | IC50 (μg/ml) |
|---|---|---|---|---|
| Hd.Cr | 1000 | 24.7 ± 0.3 | 86.4 ± 1.1∗∗∗ | 195.7 |
| 500 | 18.0 ± 0.6 | 64.3 ± 1.9∗∗∗ | ||
| 250 | 15.0 ± 1.2 | 54.4 ± 2.8∗∗∗ | ||
| 125 | 13.3 ± 0.0 | 44.6 ± 1.1∗∗∗ | ||
| 62.5 | 11.0 ± 0.3 | 39.0 ± 0.0∗∗∗ | ||
| 31.25 | 10.0 ± 0.6 | 33.5 ± 1.9∗∗∗ | ||
|
| ||||
| Hd.Hex | 1000 | 20.0 ± 0.4 | 62.3 ± 1.7∗∗∗ | 390.2 |
| 500 | 17.3 ± 0.5 | 53.4 ± 1.9∗∗∗ | ||
| 250 | 13.7 ± 1.2 | 41.2 ± 1.1∗∗∗ | ||
| 125 | 12.0 ± 0.3 | 35.7 ± 2.8∗∗∗ | ||
| 62.5 | 11.5 ± 0.9 | 33.5 ± 1.1∗∗∗ | ||
| 31.25 | 09.0 ± 0.6 | 25.7 ± 0.0∗∗∗ | ||
|
| ||||
| Hd.Chf | 1000 | 25.0 ± 1.2 | 87.5 ± 2.8∗ | 25.5 |
| 500 | 22.5 ± 0.5 | 78.8 ± 1.1∗∗ | ||
| 250 | 20.0 ± 0.0 | 71.7 ± 0.0∗∗∗ | ||
| 125 | 18.7 ± 0.3 | 66.5 ± 2.2∗∗ | ||
| 62.5 | 16.2 ± 0.6 | 59.0 ± 1.7∗∗∗ | ||
| 31.25 | 14.7 ± 0.6 | 51.0 ± 1.9∗∗∗ | ||
|
| ||||
| Hd.EA | 1000 | 25.0 ± 0.6 | 81.1 ± 1.7∗∗ | 60.3 |
| 500 | 20.5 ± 0.7 | 66.4 ± 0.2∗∗∗ | ||
| 250 | 19.0 ± 0.6 | 61.7 ± 1.9∗∗∗ | ||
| 125 | 17.6 ± 1.2 | 54.0 ± 0.2∗∗∗ | ||
| 62.5 | 16.2 ± 0.0 | 50.2 ± 2.8∗∗∗ | ||
| 32.25 | 14.4 ± 0.2 | 44.4 ± 1.8∗∗∗ | ||
|
| ||||
| Hd.Aq | 1000 | 14.0 ± 0.2 | 46.2 ± 0.4∗∗∗ | 1110.7 |
| 500 | 12.2 ± 0.4 | 42.0 ± 1.1∗∗∗ | ||
| 250 | 11.3 ± 0.7 | 38.8 ± 1.8∗∗∗ | ||
| 125 | 9.7 ± 0.9 | 30.0 ± 0.4∗∗∗ | ||
| 62.5 | 8.2 ± 0.0 | 28.2 ± 0.8∗∗∗ | ||
| 31.25 | 5.3 ± 0.5 | 17.9 ± 0.1∗∗∗ | ||
|
| ||||
| Hd.Sp | 1000 | 28.2 ± 0.8 | 92.7 ± 0.0ns | 18.3 |
| 500 | 25.7 ± 0.1 | 82.3 ± 1.9∗ | ||
| 250 | 23.0 ± 0.0 | 77.6 ± 1.2∗ | ||
| 125 | 19.5 ± 0.3 | 62.9 ± 1.9∗∗ | ||
| 62.5 | 17.1 ± 0.9 | 55.2 ± 0.0∗∗∗ | ||
| 31.25 | 16.8 ± 0.4 | 53.3 ± 0.5∗∗ | ||
Positive control was taken as vincristine sulfate having IC50value < 0.1 μg/ml; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 Hd.Cr crude methanol extract, Hd.Hex fraction of n-hexane, Hd.Chf fraction of chloroform, Hd.EA fraction of ethyl acetate, Hd.Aq fraction of aqueous layer, Hd.Sp fractions of saponins. ns: nonsignificant.