Table 5.

Concentration-dependent cytotoxicity of H. digitata crude extract and resultant fraction values against Artemia salina (brine shrimps) and their LC₅₀ values.

Samples	Total treated	Concentration dose (mg/ml)	Killed nauplii (n = 3)			Killed mean	Percent cytotoxicity	LC50 (mg/ml)
			I	II	III
Hd.Cr	30	1000	18	22	17	19.00 ± 0.58	64.1 ± 1.5	262
		100	14	15	14	14.33 ± 0.56	47.6 ± 1.9
		10	10	12	14	11.33 ± 0.52	37.4 ± 1.3
Hd.Hx	30	1000	17	18	19	18.00 ± 0.04	60.2 ± 0.4	630
		100	11	12	10	11.00 ± 0.06	36.7 ± 0.8
		10	07	08	07	07.33 ± 0.58	24.4 ± 1.9
Hd.Chf	30	1000	22	24	24	23.31 ± 0.56	77.4 ± 1.7	51
		100	17	19	17	17.66 ± 0.57	58.7 ± 2.2
		10	12	13	14	13.00 ± 0.20	43.5 ± 0.7
Hd.EA	30	1000	21	21	19	20.33 ± 0.56	67.6 ± 1.7	481
		100	11	13	11	11.67 ± 0.54	38.7 ± 1.5
		10	08	09	10	09.01 ± 0.02	30.0 ± 0.4
Hd.Aq	30	1000	18	19	20	19.00 ± 0.20	63.5 ± 0.2	420
		100	12	13	14	13.00 ± 0.04	43.1 ± 0.4
		10	08	10	10	09.33 ± 0.56	31.3 ± 1.7
Hd.Sp	30	1000	29	29	29	29.00 ± 0.20	96.5 ± 0.2	<0.01
		100	22	22	20	21.33 ± 0.56	71.3 ± 1.7
		10	17	17	18	17.67 ± 0.54	58.7 ± 1.5

Positive control (etoposide LD₅₀ 9.8). PPM = part per million; SD = standard deviation; Unbiased = s=$\sqrt{\sum {\left(x-\overline{x}\right)}^2/n}-1.$