Table 1.
List of MLST loci, primers, and amplification conditions used for the typing of pathogenic Leptospira spp, based on the method described by Boonsilp et al., 2013 with modifications.
| Loci | Primers | Nucleotide sequence (5´ a 3´) | MgCl2 (mM) | Primers concentration (μM) | PCR size (pb) | loci size (pb) | Localization in chromosome I |
|---|---|---|---|---|---|---|---|
| pntA | pntA-FM | TAG GAA ARA TGA AAC CRG GAA C | 3.5 | 0.2 | 621 | 525 | 56347–56871 |
| pntA-RM | AAG AAG CAA GAT CCA CAA YTA C | ||||||
|
| |||||||
| caiB | caiB-F | CAACTTGCGGAYATAGGAGGAG | 3.5 | 0.2 | 650 | 402 | 1562845–1563246 |
| caiB-R | ATTATGTTCCCCGTGAYTCG | ||||||
|
| |||||||
| glmU | glmU-FM | AGG ATA AGG TCG CTG TGG TA | 3.5 | 0.2 | 650 | 444 | 3784955–3784512 |
| glmU-RM | AGT TTT TTT CCG GAG TTT CT | ||||||
|
| |||||||
| tpiA | pntA-FM | TTG CAG GAA ACT GGA AAA TGA AT | 3.5 | 0.2 | 639 | 426 | 1694673–1694248 |
| pntA-RM | GTTTTACRGAACCHCCGTAGAGAAT | ||||||
|
| |||||||
| pfkB | pfk B-FM | CGGAGAGTTTTATAARAAGGACAT | 1.5 | 0.2 | 588 | 432 | 1386553–1386984 |
| pfk B-RM | AGAACACCCGCCGCAAAACAAT | ||||||
|
| |||||||
| sucA | sucA-FM | TCA TTC CAC TTY TAG ATA CGA T | 3.5 | 0.6 | 640 | 447 | 1227474–1227920 |
| sucA-RM | TCTTTTTTGAATTTTTGACG | ||||||
|
| |||||||
| mreA | mreA-FM | GGC TCG CTC TYG ACG GAA A | 3.5 | 0.6 | 719 | 435 | 2734550–2734116 |
| mreA-RM | TCCRTAACTCATAAAMGACAAAGG | ||||||