TABLE 1.
Target site sequences for IS186 insertion
| Accession no.a | Target site sequence (5′–3′)b |
|---|---|
| AL512784 | AGGGGGCG↓GGGGCAGCACCC↓CCCTCC |
| AL392173 | TGTGGGG↓TTGGTGCCCC↓CGCA |
| AL049646 | GGCG↓GGGGCTG↓CCCCCACC |
| AL080249 | AGGTG↓GGGGGCAGCCCC↓CGTCC |
| AC007999 | CGGGG↓GGCTGA↓CCCCCT |
| AL390241 | GGTGGGGGG↓CAGCCCCC↓GCCCGG |
| AL355498 | GGCG↓GGGAACATCCC↓ACCCC |
| AL445189 | GGGGGAG↓GGGGTATAA↓CCCCACCCC |
| AC006487 | CG↓GGGGGCGG↓CCCCCG |
| AC004448 | GGCG↓GGGGCTGA↓CCCCCACC |
| Z97985 | CCGGGCG↓GGGGGCTGA↓CCCCCCCACC |
| AC006137 | CGCG↓GGGCTCCG↓CCCCCGCC |
| AF211971 | TGGGGGG↓GTCAGCC↓CCCCGCC |
| AC074331 | TGGGG↓GGCAGC↓CCCCGCC |
| Z78058 | GGGCGGGG↓GTAGTGAC↓CCCGCC |
| AE000112c | AGTGGCGGGG↓ATCACT↓CCCCGCCGTT |
| AE000163c | TTGTGCGGGG↓AGTAAT↓CCCCGCATCC |
| AE000327c | AAAGCCGGGG↓ATAATT↓CCCCGGTTTT |
a
Accession numbers are taken from entries in the GenBank sequence database.
b
Vertical arrows demarcate the host sequence that underwent duplication following IS186 transposition. The runs of Gs and Cs which are postulated to define the consensus for IS186 insertion (see text) are underlined. It has been assumed that the IS186 element carries a pair of 25-bp inverted repeats at its ends (15).
c
Entry pertaining to the native IS186 insertions in the wild-type E. coli K-12 chromosome.