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Gradient eluting procedures for isolation of PFHxS isomers.
| Time (min) | Flow rate (mL/min) | A (%) aqueous phase(20 mM ammonium formate, pH = 4.0) | B (%) organic phase(methonal or acetonitrile) | Curve |
|---|---|---|---|---|
| 0.0 | 0.25 | 90 (90) | 10 (10) | 6 |
| 2.0 | 90 (90) | 10 (10) | 6 | |
| 5.0 | 40 (70) | 60 (30) | 6 | |
| 16.0 | 12 (30) | 88 (70) | 6 | |
| 16.5 | 8 (0) | 92 (100) | 6 | |
| 17.5 | 0 (0) | 100 (100) | 6 | |
| 20.0 | 90 (90) | 10 (10) | 6 | |
| 23.0 | 90 (90) | 10 (10) | 6 |
