Table 5.
Repeatability of the ELISA method for rye haylage. Two operators measured AFL B1 in three repetitions on three different days from freshly prepared samples. SD, standard deviation. Spike: 40 ppb AFL B1.
| Rye haylage | Repetition | 1st day AFL B1 (ppb) | 2nd day AFL B1 (ppb) | 3rd day AFL B1 (ppb) |
|---|---|---|---|---|
| Daily | Mean | 37.10 | 34.58 | 38.42 |
| SD | 1.95 | 3.87 | 1.72 | |
|
| ||||
| Significance test between days results | t | t crit (95%) | Result | Evaluation |
|
| ||||
| 1-2 | 1.4222 | 2.3646 | t < tcrit | No significant difference |
| 2-3 | −1.2458 | 2.2281 | t < tcrit | No significant difference |
| 1–3 | −2.2205 | 2.3646 | t < tcrit | No significant difference |
|
| ||||
| Significance test between operators | t | t crit (95%) | Result | Evaluation |
|
| ||||
| Operator 1- operator 2 | −1.8758 | 2.1447 | t < tcrit | No significant difference |