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siBRD4 inhibits the TLR4-NF-κB pathway in Ang II-induced H9c2 cells. Real-time PCR was performed to measure the mRNA level of TLR4 (a). Western blot was carried out to determine the TLR4 (b), phosphorylated NF-κB p65(c), total NF-κB p65(d), protein expression in H9c2 cells (e). Each experiment was performed in triplicate and repeated at least three times. ∗∗∗P < 0.001 vs. control group; ##P < 0.01, ###P < 0.001 vs. Ang II + siNC group.
