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. 2022 Aug 19;127(8):1461–1472. doi: 10.1038/s41416-022-01927-y

Fig. 6. IL-1β/ESE3 (PSCs)/IL-1β-positive feedback loop promoted PDAC progression.

Fig. 6

mRNA (a) and protein (b) expression levels of ESE3, α-SMA, collagen-I and IL-1β in hpPSCs and mpPSCs stimulated with recombinant human IL-1β (100 ng/mL, 24 h). c Western blot analyses of ESE3, α-SMA, collagen-I and IL-1β expression in ihPSCs and hpPSCs with IL-1β stimulation and/or knockdown of ESE3 expression. Western blot (d) and RT-PCR (e) analyses of p-p65, ESE3, α-SMA, collagen-I and IL-1β expression in ihPSCs and hpPSCs with IL-1β stimulation and/or NF-κB (p65) inhibitor Bay11 treatment. f Western blot analyses of p-p65, ESE3, α-SMA, collagen-I and IL-1β expression in ihPSCs and hpPSCs with IL-1β stimulation and/or knockdown of NF-κB (p65) expression. g Schematic of the IL-1β/NF-κB/ESE3 signalling axis that increased α-SMA, collagen-I and IL-1β expression in PSCs and promoted fibrosis and chemoresistance. *P < 0.05 and **P < 0.01.