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. 2022 Jul 28;127(8):1440–1449. doi: 10.1038/s41416-022-01916-1

Fig. 3. Significant upregulation of angiogenesis (CD31 + ve vessels) in colon adenocarcinoma shows a strong positive correlation with NPY expression.

Fig. 3

a Representative images of immunohistochemistry showed significantly increased angiogenesis in human colon adenocarcinoma (CA) (non-metastatic and metastatic) compared to the normal colon. n = 21 per group. Scale bar, 100 μm. b Graphical representation of CD31 + ve microvessels in ten high-power microscopic fields (HPF) of normal human colon, non-metastatic and metastatic CA tissues. c Correlation between NPY IHC score and CD31 counts in colon tissues (r2 = 0.8397). d Significant upregulation of angiogenesis (CD31 + ve vessels) in orthotopic HT29 tumours. e Graphical representation of CD31 + ve microvessels in ten HPF of HT29 tumours and normal colon. Data represent the summary of six or more sections per mouse from each group. f Correlation between NPY IHC score and CD31 counts in HT29 tumours (r2 = 0.8175). g Representative confocal microscopy images showed high expression of Y2R (red) on CD31 + ve endothelial cells (Green) of blood vessels in human metastatic CA (scale bar, 80 μm; n = 21) and h orthotopic HT29 colon tumours (scale bar, 80 μm, n = 11). Results are expressed as mean ± SEM; *P < 0.01 compared to the normal colon.