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. 2022 Oct 11;8:106. doi: 10.1038/s41421-022-00447-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Heatmap of the transcription levels (left) and protein levels (right) of genes related to ketone body metabolism in E18.5d, P1d and P7d mouse hearts according to the transcriptomics, proteomics and metabolomics data. b qPCR detection of Hmgcs2 mRNA levels in the hearts of E18.5d, P1d and P7d mice (n = 5 for each group). c qPCR detection of Oxct1 mRNA levels in the hearts of E18.5d, P1d and P7d mice (n = 13 for each group). d Relative β-HB levels in E18.5d, P1d and P7d mouse hearts according to the metabolomics data (n = 3 for each group). e Western blot detection of the protein levels of HMGCS2 in multiple tissues of mice from the embryonic period to adulthood. f β-HB levels in mouse hearts from the embryonic period to adulthood (n = 3–6 for each group). g Blood β-HB levels of mice from the embryonic period to adulthood (n = 5–16 for each group). h Western blot detection of the protein levels of OXCT1 in mouse hearts from the embryonic period to adulthood. Data are presented as means ± SEM. and the P values were determined by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.