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. 2022 Oct 11;8:106. doi: 10.1038/s41421-022-00447-6

Fig. 3. FA in colostrum stimulates transiently abundant Hmgcs2 expression in neonatal mouse heart.

Fig. 3

a Western blot was performed to detect the expression levels of HMGCS2 in hearts from WT and Cidea−/− mouse offsprings (P3d). b Hmgcs2 mRNA levels of NRVMs treated with FA (200 μM) for different times (n = 4 for each group). c Protein levels of HMGCS2 in NRVMs treated with FA (200 μM) for different times. d Hmgcs2 mRNA levels in NRVMs treated with different doses of FA for 24 h (n = 4 for each group). e Protein levels of HMGCS2 in NRVMs treated with different doses of FA for 24 h. f NRVMs were treated with FA (200 μM) for 24 h, and then the FA in the medium was removed. Then, NRVMs were cultured for 2 days (2d), 4 days (4d), and 6 days (6d), and western blotting was performed to detect HMGCS2 protein levels. g Oxct1 mRNA levels of NRVMs treated with FA (200 μM) for different times (n = 6 for each group). h Protein levels of OXCT1 in NRVMs treated with FA (200 μM) for different times. i Oxct1 mRNA levels in NRVMs treated with different doses of FA for 24 h (n = 6 for each group). j Protein levels of OXCT1 in NRVMs treated with different doses of FA for 24 h. Data are presented as means ± SEM. and the P values were determined by one-way ANOVA. **P < 0.01; ***P < 0.001.