Figure 6.

PGM3 enhances β-catenin activity and accelerates cell proliferation by elevating O-GlcNAcylation level. (A) Non-specific siRNA or PGM3 siRNA was transfected into HCT15 cells. 48 h after transfection, cells were harvested, and O-GlcNAc protein level was measured using western blotting. (B) PGM3 plasmid was transfected into SW480 cells, which were then treated with or without 30μM OMSI-1 for 24 h, O-GlcNAc protein level was measured using western blotting. (C) PGM3 plasmid was transfected into SW480 cells, which were then treated with or without 30μM OMSI-1 for 24 h, β-catenin, c-Myc, and cyclin D1 protein levels were measured by western blotting. (D) PGM3 plasmid was transfected into SW480 cells, which were then treated with or without 30μM OMSI-1, CCK-8 was used to examine cell proliferation. (E) PGM3 siRNA was transfected into HCT15 cells, which were then treated with or without 50 mM UDP-GlcNAc for 24 h, O-GlcNAc level was analyzed by western blotting. (F) PGM3 siRNA was transfected into HCT15 cells, which were then treated with or without 50 mM UDP-GlcNAc, CCK-8 was used to examine cell proliferation. (A color version of this figure is available in the online journal.)