| Critical commercial assays |
|
| COVIDSeq Assay (96 samples) |
Illumina |
Cat# 20049393 |
| COVIDSeq v4 Primer Pools |
Illumina |
Cat# 20065135 |
| Quick-RNA Viral 96 Kit |
Zymo Research |
Cat# R1041 |
| MagMAX™ Viral/Pathogen II (MVP II) Nucleic Isolation Kit |
ThermoFisher |
Cat# A48383 |
| Applied Biosystems TaqPath Master Mix no ROX |
ThermoFisher |
Cat# A28523 |
| CDC 2019-nCoV Real-Time RT-PCR Primers and Probes |
IDT |
Custom; see (Landaverde et al., 2022) |
|
| Deposited data |
|
| Raw sequencing data |
NCBI Bioproject |
Bioproject: PRJNA867488 |
| Community consensus sequences |
GISAID EPI_SET |
EPI_SET: EPI_SET_220804bm |
|
| Software and algorithms |
|
| Bowtie2 |
bowtie-bio.sourceforge.net |
v2.3.4.1 |
| Samtools |
htslib.org |
v1.15.1 |
| LoFreq |
csb5.github.io/lofreq |
v2.1.3.1 |
| R |
r-project.org |
v4.0.2 |
| Pangolin |
pangolin.cog-uk.io |
v4.1.1 |
| Pango data |
pangolin.cog-uk.io |
v1.11 |
| tidyverse |
tidyverse.org |
v1.3.0 |
| argparse |
github.com/trevorld/r-argparse |
v2.1.5 |
| BEAST |
github.com/beast-dev/beast-mcmc |
v1.10.4 |