TABLE 2.
Plasmids used in this study
| Plasmid | Phenotype/genotype | Description | Source |
|---|---|---|---|
| pKSII+ | bla ColE1 | Stratagene | |
| pSKII+ | bla ColE1 | Stratagene | |
| pCR2.1 | bla aph ColE1 | Invitrogen | |
| pMV261.kan | aph Phsp60 oriM ColE1 | Multicopy mycobacterial shuttle plasmid | 46 |
| pMV306.kan | aph int attP ColE1 | Single-copy mycobacterial shuttle plasmid | 46 |
| pMV361.hyg | hyg Phsp60int attP ColE1 | Single-copy mycobacterial shuttle plasmid | 46 |
| pYUB412 | bla hyg int attP ColE1 cosλ | Single-copy mycobacterial shuttle plasmid | S. Bardarov and W. R. Jacobs |
| pYUB282 | bla ColE1 cosλ | V. Balasubramanian and W. R. Jacobs | |
| pMD31 | aph oriM ColE1 | Multicopy mycobacterial shuttle plasmid | 12 |
| pMB198 | hyg int attP ColE1 | Phsp60 deleted from pMV361.hyg | This work |
| pYUB657 | bla hyg Phsp60-sacB ColE1 | Counterselectable suicide vector for mycobacteria | 37 |
| pYUB495 | bla secA1 (M. smegmatis) ColE1 cosλy | pYUB282 containing M. smegmatis secA1 | This work |
| pYUB536 | bla secA1 (M. smegmatis) ColE1 | 4.0-kb BamHI fragment containing M. smegmatis secA1 from pYUB495 cloned in pKSII+ | This work |
| pYUB544 | aph Phsp60-secA1 oriM ColE1 | 4.0-kb BamHI fragment from pYUB536 cloned into pMV261; M. smegmatis secA1 under control of hsp60 promoter | This work |
| pH.3 | bla secA1 (M. bovis BCG) ColE1 | pKSII+ containing an 8.0-kb fragment with M. bovis BCG secA1 | This work |
| pYUB499 | aph secA1 (M. bovis BCG) oriM ColE1 | 3.0-kb HindIII-KpnI fragment of M. bovis BCG secA1 cloned into pMD31 | This work |
| pYUB538 | aph secA1AzR (M. bovis BCG) oriM ColE1 | pYUB499 with secA1 azide resistance allele | This work |
| pMB117 | bla secA1 (M. smegmatis) ColE1 | 12.0-kb SacI fragment containing M. smegmatis secA1 from pYUB495 cloned into pKSII+ | This work |
| pMB120 | bla ΔsecA1 (M. smegmatis) ColE1 | In-frame deletion mutant form of secA1 derived from pMB117 | This work |
| pMB125 | bla ΔsecA1 (M. smegmatis) ColE1 | SacI fragment of pMB120 subcloned into pSKII+ | This work |
| pMB128 | bla hyg Phsp60-sacB ΔsecA1 (M. smegmatis) ColE1 | ΔsecA1 from pMB125 cloned into pYUB657 | This work |
| p6-1 | bla secA2 (M. smegmatis) ColE1 | pYUB412 cosmid containing M. smegmatis secA2 | This work |
| pMB144 | bla aph secA2 (M. tuberculosis) ColE1 | M. tuberculosis secA2 cloned into pCR2.1 | This work |
| pMB147 | aph Phsp60-secA2 (M. tuberculosis) oriM ColE1 | M. tuberculosis secA2 from pMB144 cloned into pMV261 under control of Phsp60 | This work |
| pMB148 | bla secA2 (M. smegmatis) ColE1 | 6.0-kb BamHI fragment from p6-1 containing M. smegmatis secA2 cloned into pSKII+ | This work |
| pMB155 | bla secA2 (M. smegmatis) ColE1 | 3.2-kb BamHI-ScaI fragment of pMB148 carrying M. smegmatis secA2 cloned into pKSII+ | This work |
| pMB156 | bla ΔsecA2 (M. smegmatis) ColE1 | In-frame deletion of secA2 derived from pMB155 | This work |
| pMB160 | bla hyg Phsp60-sacB ΔsecA2 (M. smegmatis) ColE1 | ΔsecA2 cloned from pMB156 into pYUB657 | This work |
| pMB162 | aph Phsp60-secA2 (M. tuberculosis) int attP ColE1 | HindIII-XbaI fragment carrying Phsp60-secA2 from pMB147 subcloned into pMV306.kan | This work |
| pMB110 | aph Phsp60-′phoA oriM ColE1 | ′phoA PCR product cloned under control of Phsp60 | This work |
| pMB174 | aph Phsp60-′phoA int attP ColE1 | NotI fragment of pMB110 carrying Phsp60-′phoA cloned into pMV306.kan | This work |
| pMB175 | aph secA1 (M. bovis BCG) int attP ColE1 | HindIII/Asp718 M. bovis BCG secA1 containing fragment cloned into pMV306.kan | This work |
| pMB196 | aph pepA::Tn552 ′phoA oriE1 hyg int attP ColE1 | pepA::′phoA fusion recovered as NheI fragment from mc22724 cloned into pMB198 | This work |
| pMB202 | hyg rv1566c::Tn552 ′phoA oriE1 kan int attP ColE1 | rv1566c::′phoA fusion recovered as NarI fragment from mc22725 cloned into pMB198 | This work |
| pMB203 | hyg fbpB::Tn552 ′phoA oriE kan int attP ColE1 | fbpB::′phoA fusion recovered as NarI fragment from mc22718 cloned into pMB198 | This work |
| pMB206 | hyg fbpB-HA::Tn552 ′phoA oriE1 kan int attP ColE1 | HA tag cloned in frame into fbpB::′phoA fusion in pMB203 | This work |
| pMB207 | bla aph secA2 (M. smegmatis) ColE1 | M. smegmatis secA2 cloned into pCR2.1 | This work |
| pMB208 | aph Phsp60-secA2 (M. smegmatis) oriM ColE1 | M. smegmatis secA2 from pMB207 cloned into pMV261 under control of Phsp60 | This work |