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Secondary recombination products produced from single-crossover strains
| Strain (plasmid) | % secA allele present
among secondary recombinants
|
na | |
|---|---|---|---|
| Deletion | Wild type | ||
| secA1 single crossover | |||
| MB526 | 0 | 100 | 13 |
| MB526(pMV261) | 0 | 100 | 9 |
| MB526(pYUB544) | 62 | 38 | 16 |
| MB526(pYUB499) | 59 | 41 | 39 |
| MB526(pMB208) | 0 | 100 | 20 |
| MB526(pMB147) | 0 | 100 | 24 |
| MB526(pMB162) | 0 | 100 | 19 |
| secA2 single crossover | |||
| MB573 | 31 | 69 | 13 |
| MB575 | 50 | 50 | 18 |
Number of sucrose-resistant and hygromycin-sensitive recombinants analyzed.
