TABLE 1.
Target genes and reference gene for the comparative gene transcription analysis in osteoblasts derived from alveolar and iliac crest bone (Bonewald, 2011; Stephens et al., 2011; Bellido, 2014). Primer Assay information are listed in Supplementary Table S2.
| Gene | Description | Expressed in | Function |
|---|---|---|---|
| RUNX2 | Runt related transcription factor 2 | Osteoblasts | Osteogenic differentiation |
| PDPN | Podoplanin | Early osteocytes | Formation of dendrites |
| PHEX | Phosphate regulating endopeptidase homolog, X-linked | Early and mature osteocytes | Phosphate metabolism |
| COL1A1 | Collagen type 1 alpha 1 chain | Osteoblasts | Bone matrix formation |
| BGLAP | Bone gamma-carboxyglutamate protein | Osteoblasts | Mineralization |
| SPP1 | Secreted phosphoprotein 1 | Osteoblasts Osteocytes | Mineralization; adhesion of osteoclasts to mineralized ECM |
| ALPL | Alkaline phosphatase liver/bone/kidney | Osteoblasts | Mineralization |
| OPG | TNF receptor superfamily member 11b | Osteoblasts, Osteocytes | Inhibitor of osteoclastogenesis |
| CSF1 | Colony stimulating factor 1 | Osteoblasts, osteocytes | Proliferation and survival of osteoclasts and osteoclast precursor cells |
| EPHB4 | EPH receptor B4 | Osteoblasts | Inhibits osteoclast activity; stimulates osteogenic differentiation |
| SEMA3A | Semaphorin 3A | Osteoblasts | Inhibits osteoclastogenesis; stimulates osteogenic differentiation |
| VDR | Vitmin D receptor | Osteoblasts Osteocytes | Positive regulator of bone resorption; facilitates Ca-absorption |
| HPRT1 | Hypoxanthine phosphoribosyltransferase 1 | Osteoblasts Osteocytes | Purine synthesis through the purine salvage pathway |