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. 2022 Apr 20;11(19):3687–3699. doi: 10.1002/cam4.4745

FIGURE 4.

FIGURE 4

ADSCs‐derived exosomes miR‐138‐5p suppresses BC cells migration, invasion and proliferation in vitro. (A–D) The biological role of ADSCs‐exo miR‐138‐5p on cell migration, invasion, and proliferation capability were assessed by wound healing, transwell matrigel invasion assay and CCK‐8 assays in 5637 and T24. ADSCs‐exo miR‐138‐5p of 50 μg/ml was used as the experimental concentration. Wound healing assay, scale bar, 100 μm. Transwell invasion assay, scale bar, 50 μm. *p < 0.05, **p < 0.01. (E) After BC cells cocultured with ADSCs‐exo miR‐138‐5p, the expression level of survivin was determined by western blot analysis. β‐Actin used as internal reference. *p < 0.05. (F–I) After BC cells cocultured with ADSCs‐exo miR‐138‐5p inhibitor, the cells migration, invasion, and proliferation abilities were evaluated by wound healing assay, transwell matrigel invasion assay and CCK‐8 assays. ADSCs‐exo miR‐138‐5p of 50 μg/ml was used as the experimental concentration. Wound healing assay, scale bar, 100 μm. Transwell invasion assay, scale bar, 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001. (J) The expression level of survivin was determined by western blot analysis. β‐Actin used as internal reference. *p < 0.05