FIGURE 1.

PIEZO1 is identified as a potential ADC target by high‐throughput membrane proteome screening. (A) The schematic diagram for the whole study design; (B) The quality control of membrane‐anchored proteins' enrichment derived from ESCC patients' samples, GAPDH was used for defining cytosol proteins and ATBSB was for membrane components; (C) Overlapping of overexpressed membrane protein candidates between and among patients; (D) Internalization assay by immunofluorescence using PIEZO1 antibody in TE‐1 cells (scale bar: 20 μm); (E) Internalization efficiency evaluation of PIEZO1 antibody into TE‐1 cells with flow cytometry quantification of PIEZO1 immunofluorescence; (F) Cytotoxicity test of Anti‐PIEZO1‐MMAE in TE‐1 cells, Anti‐PIEZO1 antibody, and IgG‐MMAE were used as vehicle and isotype control, respectively