FIGURE 3.

The cytotoxicity of Anti‐PIEZO1‐MMAE is the dosage and cell surface PIEZO1 expression dependent. (A, B) Transcription and protein level of PIEZO1 in human ESCC cell lines TE‐1, Eca109, and Kyse150. ACTB was used as an internal control. (C) In situ immunofluorescence staining assay of PIEZO1 in TE‐1, Eca109, and Kyse150, scale bar: 100 μm; (D) Microscopic assay of cell morphology of TE‐1, Eca109, and Kyse150 treated with different dosages of Anti‐PIEZO1‐MMAE (1, 5, and 10 nM) for 48 h; IgG‐MMAE was used as isotype control, scale bar: 100 μm; (E) Cell viability assay via CCK‐8 test of TE‐1, Eca109, and Kyse150 treated with different dosage Anti‐PIEZO1‐MMAE (1, 5, and 10 nM) for 48 h; data were normalized to empty control