TABLE 1.

Primers employed in the construction of plasmid pM2VegCEGFDnaBcba2.

Primer a	Sequence b
P1: EcoRI-VegC F	5′-GGG​gaa​ttc​TAA​TTT​AAA​TTT​TAT​TTG​ACA​AAA​ATG​GGC​TCG​TGT​TGT​CCA​ATA​AAT​GT-3′
P2: VegC-lacO-RBS R	5′-CAT​TTT​TAT​CAC​CTC​CTT​TGT​GAA​ATT​GTT​ATC​CGC​TCA​CAA​TTC​CAC​CTC​ACT​ACA​TTT-3′
P3: RBS-EGF F	5′-GAG​GTG​ATA​AAA​ATG​AAT​AGT​GAC​TCT​GAA​TGT​CCC​CTG​TCC​CAC​GAT​GGG​TAC​TGC​CTC-3′
P4: DnaB-cba2 R	5′-CGC​CTC​GCC​GTC​CGG​GTC​GTC​GCC​TGC​GTT​GTG​TAC​AAT​GAT​GTC​ATT​CGC​GAC​AAA-3′
P5: cba2 F	5′-GCG​AGG​CGG​CCG​TCA​CCG​AGC​GGC​TGC​AGC​AGG​GGC​GGT​CGC​TGC​CG-3′
P6: cba2-StuI R	5′-CGG​Aag​gcc​tCG​TCG​CCT​GCC​ACC​GTC​CCG​TAC​TGC​TCC​GCG-3′

^aF and R stand for forward and reverse, respectively.

^bRestriction sites used in the cloning experiments are shown in lowercase.