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. 2022 Sep 28;13:1000737. doi: 10.3389/fmicb.2022.1000737

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Copyright © 2022 Gonçalves, Santos, Gomes, Armengaud, Miragaia and Coelho.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PLS-DA model based on metabolites concentrations for the three pH conditions. (A) PLS-DA scores plot of first and second component, showing the discrimination between the 3 experimental conditions, N55 (green) N57 (yellow), and N77 (red) (with 5 components: R² = 0.97 and Q² = 0.92) using the metabolites levels. Metabolites that most contribute for the separation of the groups based on its VIP scores (>1) for (B) the first component and, (C) the second component; the colour scale presents the metabolite relative levels among the three experimental conditions.