TABLE 1.
Identified metabolites from S epidermidis 19N strain intracellular extract by 1H-NMR with respective levels in each experimental condition.
| Metabolites | N55 |
N57 |
N77 |
N55/N77 |
N55/N57 |
N57/N77 |
|||
| Mean (nmol) |
SD (nmol) |
Mean (nmol) |
SD (nmol) |
Mean (nmol) |
SD (nmol) |
Fold change |
Fold change |
Fold change |
|
| Acetate | 60.7 | 9.1 | 59.0 | 13.0 | 134.3 | 28.6 | 0.5*** | 1.0 | 0.4*** |
| Adenine | 11.1 | 7.0 | 8.3 | 4.2 | 15.4 | 5.8 | 0.7 | 1.3 | 0.5* |
| Alanine | 44.6 | 19.3 | 27.2 | 13.9 | 51.4 | 19.6 | 0.9 | 1.6 | 0.5* |
| Asparagine | 32.7 | 15.9 | 74.1 | 36.7 | 93.5 | 16.6 | 0.4*** | 0.4* | 0.8 |
| Aspartate | 142.5 | 25.0 | 220.0 | 41.4 | 434.3 | 69.2 | 0.3*** | 0.6** | 0.5*** |
| Betaine | 1326.8 | 171.8 | 402.5 | 237.3 | 1254.2 | 149.8 | 1.1 | 3.3*** | 0.3*** |
| Choline | 32.0 | 7.6 | 4.5 | 1.4 | 15.2 | 1.5 | 2.1*** | 7.0*** | 0.3*** |
| Cystathionine | 8.3 | 2.1 | 25.9 | 9.5 | 27.3 | 2.8 | 0.3*** | 0.3*** | 0.9 |
| Cytidine monophosphate | 17.6 | 12.2 | 12.3 | 9.7 | 26.9 | 8.0 | 0.7 | 1.4 | 0.5** |
| Glutamate | 807.3 | 99.7 | 442.3 | 125.3 | 651.0 | 43.4 | 1.2*** | 1.8*** | 0.7*** |
| Glycine | 3.7 | 3.2 | 21.3 | 34.8 | 2.4 | 3.0 | 1.5 | 0.2 | 8.9* |
| Guanosine | 5.5 | 2.2 | 4.0 | 3.5 | 6.2 | 1.9 | 0.9 | 1.4 | 0.6* |
| Histidine | 4.2 | 1.4 | 3.6 | 0.5 | 5.4 | 1.0 | 0.8 | 1.2 | 0.7** |
| Isoleucine | 3.2 | 1.4 | 3.8 | 1.6 | 6.1 | 0.8 | 0.5*** | 0.8 | 0.6** |
| Isovalerate | 3.4 | 2.2 | 1.4 | 0.9 | 4.1 | 0.9 | 0.8 | 2.5 | 0.3** |
| Lactate | 19.6 | 12.2 | 16.5 | 6.7 | 61.4 | 18.3 | 0.3*** | 1.2 | 0.3*** |
| Leucine | 16.8 | 4.4 | 16.1 | 7.0 | 25.4 | 5.0 | 0.7** | 1.0 | 0.6* |
| Lysine | 20.9 | 8.2 | 16.3 | 4.0 | 25.4 | 5.3 | 0.8 | 1.3 | 0.6** |
| NADP+ | 2.2 | 0.4 | 3.7 | 1.6 | 2.6 | 0.4 | 0.8 | 0.6** | 1.4* |
| Phenylalanine | 9.3 | 1.3 | 9.0 | 2.5 | 13.2 | 0.8 | 0.7*** | 1.0 | 0.7** |
| Phosphoenolpyruvic acid | 11.2 | 2.4 | 18.0 | 7.0 | 10.7 | 5.0 | 1.1 | 0.6* | 1.7* |
| sn-Glycero-3-phosphocholine | 112.6 | 24.3 | 26.2 | 12.8 | 31.3 | 3.3 | 3.6*** | 4.3*** | 0.8 |
| Succinate | 18.6 | 8.2 | 10.6 | 2.9 | 18.5 | 2.6 | 1.0 | 1.8 | 0.6** |
| Tyrosine | 1.5 | 0.6 | 1.3 | 0.5 | 2.7 | 0.7 | 0.5* | 1.1 | 0.5** |
| Uracil | 9.3 | 7.6 | 3.7 | 3.6 | 11.1 | 3.0 | 0.8 | 2.5 | 0.3** |
| Valine | 7.3 | 0.9 | 6.0 | 2.4 | 10.5 | 1.4 | 0.7*** | 1.2 | 0.6** |
| β-Alanine | 9.8 | 4.5 | 6.3 | 2.8 | 11.2 | 4.1 | 0.9 | 1.5 | 0.6* |
| 2-Hydroxyisobutyrate | 2.5 | 1.6 | 1.2 | 0.5 | 2.0 | 0.7 | 1.3 | 2.0 | 0.6 |
| 3-Hydroxyisovalerate | 6.7 | 4.6 | 4.7 | 0.9 | 4.8 | 3.5 | 1.4 | 1.4 | 1.0 |
| Adenosine | 24.4 | 10.8 | 19.0 | 13.3 | 29.7 | 12.0 | 0.8 | 1.3 | 0.6 |
| AMP | 5.7 | 1.7 | 6.8 | 5.0 | 7.5 | 5.1 | 0.8 | 0.8 | 0.9 |
| Arabinose | 17.3 | 4.5 | 13.5 | 7.1 | 16.4 | 4.7 | 1.1 | 1.3 | 0.8 |
| Coenzyme A | 5.5 | 1.9 | 12.1 | 6.7 | 11.9 | 9.1 | 0.5 | 0.5 | 1.0 |
| Formate | 94.8 | 65.7 | 36.5 | 21.0 | 67.2 | 54.8 | 1.4 | 2.6 | 0.5 |
| Glucose | 11.3 | 9.6 | 68.0 | 108.4 | 2.7 | 1.1 | 4.3 | 0.2 | 25.6 |
| Glutamine | 80.1 | 55.4 | 37.6 | 17.0 | 62.1 | 17.7 | 1.3 | 2.1 | 0.6 |
| NAD+ | 15.8 | 2.2 | 18.0 | 3.0 | 15.3 | 1.1 | 1.0 | 0.9 | 1.2 |
| Nicotinate | 2.1 | 1.0 | 1.1 | 0.4 | 1.6 | 0.4 | 1.3 | 1.9 | 0.7 |
| Sucrose | 7.0 | 10.3 | 84.5 | 75.6 | 5.5 | 7.1 | 1.3 | 0.1 | 15.3 |
| Threonine | 11.5 | 7.1 | 8.9 | 5.5 | 7.7 | 1.1 | 1.5 | 1.3 | 1.2 |
| Tryptophan | 1.4 | 0.5 | 2.0 | 0.6 | 2.3 | 1.1 | 0.6 | 0.7 | 0.9 |
| UMP | 5.9 | 3.8 | 5.2 | 3.2 | 8.3 | 3.4 | 0.7 | 1.1 | 0.6 |
| Uridine | 5.7 | 3.2 | 5.1 | 3.3 | 6.4 | 4.1 | 0.9 | 1.1 | 0.8 |
In the first (white background) and second section (grey background) are identified, the metabolites with levels significantly different (p-value ≤ 0.05), at least in one comparison, test, respectively. The fold change and p-value for each comparison in study are shown in the last 3 columns. and the non-differential by non-parametric Wilcoxon. *p-value ≤ 0.05; **p-value ≤ 0.01; ***p-value ≤ 0.001.