TABLE 2.
Functional analysis of differentially abundant proteins between N55 and N77 conditions.
| Increased at pH 5.5 | ||
| Protein | ID | Gene |
|
| ||
| Glycerolipids pathway | ||
| Glycerate kinase | SERP0409 | garK |
| Aldehyde dehydrogenase | SERP1729/2084 | aldA |
| Glycerol dehydrogenase | SERP2346 | gldA |
| Dihydroxyacetone kinase | SERP2344/5 | |
| Triacylglycerol lipase | SERP2388 | geh-1 |
| Processive diacylglycerol beta-glucosyltransferase | SERP0606 | ypfP |
| Oxidative phosphorylation/Oxidative stress | ||
| Lactaldehyde dehydrogenase | SERP2080 | |
| Alcohol dehydrogenase, zinc-containing | SERP1785/6 | |
| Pyruvate oxidase | SERP2115 | |
| Catalase | SERP0903 | katA |
| Glutathione peroxidase | SERP0872 | gpxA-1 |
| Phosphate metabolism | ||
| Polyphosphate kinase | SERP2047 | ppk |
| Exopolyphosphatase | SERP2046 | |
| Betaine Biosynthesis | ||
| Oxygen-dependent choline dehydrogenase | SERP2176 | betA |
| Betaine aldehyde dehydrogenase | SERP2177 | betB |
| Folate Biosynthesis | ||
| 2-Amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase | SERP0155 | folK |
| Dihydropteroate synthase | SERP0153 | folP |
| GTP cyclohydrolase 1 | folE | |
| Glutamate Metabolism | ||
| Glutamate synthase | SERP0109 | gltB |
| Formimidoylglutamase | SERP1919 | hutG |
| Cell Division | ||
| N-acetylmuramoyl-L-alanine amidase | SERP2263 | |
| Essential cell division protein | SERP0745 | ftsL |
| Two-component system WalK/WalR, regulator of histidine kinase | SERP2531 | YycI |
| ClpXP adapter protein | SERP0581 | SpxH |
| Peptidoglycan synthesis | ||
| Aminoacyltransferase FemA | femA | |
| Drug Transport | ||
| Drug resistance transporter | SERP1944 | |
| Drug transporter | SERP1945 | |
|
| ||
|
Increased at pH 7.4
| ||
| Protein | ID | Gene |
|
| ||
| Purine/Nucleotide Metabolism | ||
| Xanthine phosphoribosyltransferase | SERP0067 | xpt |
| Adenylate kinase | SERP1810 | adk |
| N5-carboxyaminoimidazole ribonucleotide synthase | SERP0650 | purK |
| Phosphoribosylformylglycinamidine synthase subunit PurL | SERP0654 | purL |
| Phosphoribosylformylglycinamidine cyclo-ligase | SERP0656 | purM |
| Phosphoribosylaminoimidazole-succinocarboxamide synthase | SERP0651 | purC |
| Phosphoribosylamine-glycine ligase | SERP0659 | purD |
| Phosphoribosylformylglycinamidine synthase | SERP0653 | purQ |
| Amidophosphoribosyltransferase | SERP0655 | purF |
| Glycolysis/Pyruvate metabolism | ||
| Glyceraldehyde-3-phosphate dehydrogenase | SERP1250 | gapA-2 |
| Fructose-bisphosphate aldolase | SERP1732 | fbaA |
| Pyruvate, phosphate dikinase | SERP1129 | ppdK |
| Pyruvate, water dikinase | SERP2169 | |
| Phosphoenolpyruvate carboxykinase | SERP1353 | pckA |
| Alcohol dehydrogenase | SERP0257 | adh |
| Acetate kinase | SERP1275 | ackA |
| Pyruvate formate lyase activating enzyme | SERP2365 | pflA |
| Formate acetyltransferase | SERP2366 | pflB |
| Dihydrolipoyl dehydrogenase | SERP2327 | |
| Dihydrolipoamide acetyltransferase | SERP2324 | |
| Oxidative Phosphorylation | ||
| Cytochrome aa3-600 menaquinol oxidase subunit ii | SERP0646 | qoxB |
| Cytochrome aa3-600 menaquinol oxidase subunit iii | SERP0644 | qoxC |
| Succinate dehydrogenase / fumarate reductase, cytochrome b subunit | SERP0730 | sdhC |
| NADH:flavin oxidoreductase/fumarate reductase, flavoprotein subunit | SERP2381 | |
| Butanoate metabolism | ||
| Acetoin(diacetyl) reductase | SERP2257 | budC |
| Diacetyl reductase [(S)-acetoin forming] | SERP2379 | butA |
| Acetoin dehydrogenase | SERP2325 | acoA |
| Acetoin dehydrogenase | SERP2326 | acoB |
| Acetyl-CoA C-acetyltransferase | SERP0220 | vraB |
| Arginine/Proline metabolism | ||
| Pyrroline-5-carboxylate reductase | SERP1065 | proC |
| Proline dehydrogenase | SERP1324 | putA |
| Nitric oxide synthase oxygenase | SERP1451 | |
| Ornithine carbamoyltransferase; | SERP2351 | arcB-2 |
| Carbamate kinase | SERP2352 | arcC |
| Delta-1-pyrroline-5-carboxylate dehydrogenase | SERP2128 | rocA |
| Phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) | ||
| Fructose-specific IIABC components | SERP2260 | |
| Sucrose-specific IIBC components | SERP1900/68 | |
| Glucose-specific EIICBA component | SERP2114 | ptsG |
| 6-Phospho-beta-galactosidase | SERP1789 | lacG |
| Tagatose 1,6-diphosphate aldolase | SERP1792 | lacD |
| Membrane Transporters: Hemin, Iron and Mn | ||
| Multidrug resistance efflux pump | SERP1767 | SepA |
| Flotillin-like protein | SERP1140 | FloA |
| RND family efflux transporter | SERP0014 | |
| ABC transporter, substrate-binding protein | SERP0290 | ArsC1 |
| Transferrin receptor | SERP0949/403 | |
| Iron compound ABC transporter, ATP-binding protein | SERP0402 | |
| Hemin import ATP-binding protein | SERP0015 | HrtA |
| Hemin transport system permease protein | SERP0016 | HrtB |
| Iron transporter | SERP1139 | |
| Siderophore synthetase | SERP1779 | |
| Staphyloferrin B biosynthesis | SERP1781 | |
| Extracellular Proteases/Cell Adhesion | ||
| Glutamyl endopeptidase | SspA | |
| Staphopain A Extracellular cysteine protease | sspB | |
| SdrG protein | SERP0207 | SdrG |
| Regulators of virulence factors expression | ||
| Transcriptional regulator, AraC family | SERP1972 | |
| Two Component Signal Transduction System PhoPR, Sensory box histidine kinase | SERP1255 | phoR |
| Two Component Signal Transduction System SaeRS, Response regulator | SERP0365 | saeR |
| Staphylococcal Accessory Regulator family | SERP1849/76/79 | SarR/Z/V |
| t-RNA modification | ||
| NADPH-dependent 7-cyano-7-deazaguanine reductase | SERP0394 | queF |
| Queuine trna-ribosyltransferase | SERP1203 | tgt |
| Epoxyqueuosine reductase QueH | SERP2147 | queH |
| tRNA (guanine-N(1)-)-methyltransferase | SERP0806 | trmD |