TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Reference |
|---|---|---|
| Rhizobium etli strains | ||
| CFN42 | Wild-type strain | 27 |
| CE3 | Strr derivative of CFN42 | 23 |
| CFNX89 | Derivative of CE3 cured of plasmid pCFN42d | 4 |
| CFNX636 | CE3 derivative, fixL::loxP Sp | 13 |
| CFNX637 | CE3 derivative, fixKf::ΩKm | 13 |
| CFNX642 | CFNX636 derivative, fixL::loxP | This study |
| IBTOL12 | CE3 derivative, fnrNd::ΩKm | This study |
| IBTOL14 | CE3 derivative, fnrNchr::ΩSp | This study |
| IBTOL15 | IBTOL12 derivative, fnrNd::ΩKm fnrNchr::ΩSp | This study |
| IBTOL16 | CFNX642 derivative, fixL::loxP fnrNchr::ΩSp | This study |
| IBTOL17 | CFNX642 derivative, fixL::loxP fnrNd::ΩKm | This study |
| IBTOL18 | CFNX642 derivative, fixL::loxP fnrNchr::ΩSp fnrNd::ΩKm | This study |
| Rhizobium leguminosarum bv. viciae VF39 | Smr | 8 |
| Escherichia coli strains | ||
| XL1-Blue | recA1 endA1 gyrA96 hsdR17 supE44 lac [F′ proAB lacIqlacZΔM15 Tn10 (Tcr)] thi | 6 |
| S17-1 | F−pro-82 thi-1 endA1 hsdR17 supE44 recA13, chromosomally integrated RP-4-2 (Tc::Mu) (Km::Tn7) | 31 |
| Plasmids | ||
| pBluescript SK+ | Apr, sequencing vector | Stratagene |
| pRK2013 | ColE1 mob+ Tra+ (RK2) Kmr | 10 |
| pMP220 | Transcriptional lacZ fusion vector, Tcr | 34 |
| pJQ200 SK+ | GmrsacB, suicide vector | 26 |
| pOL17 | pJQ200 derivative, fnrNd::ΩKm | This study |
| pOL18 | pJQ200 derivative, fnrNchr::ΩSp | This study |
| pJMS8 | pRK7813::Cre, Tcr | Martínez-Salazar, unpublished data |
| pOLfix10 | pMP220 derived, fixNd-lacZ gene fusion | 33 |
| pOL15 | pMP220 derived, fnrNd-lacZ gene fusion | This study |
| pOL16 | pMP220 derived, fnrNchr-lacZ gene fusion | This study |