Figure 6.

AMPK promotes GPX4-dependent ferroptosis partly through upregulation of p53. (a) Western blotting assay shows the expression of P53 and GPX4 after indicated treatments. (b) qRT-PCR presents the mRNA levels of GPX4 after indicated treatments. (c–f) The measurements of cell death, GSH, MDA, and cellular iron in cells with indicated treatments. Independent experiments were repeated three times, and representative data were shown. Data shown represent mean ± SD from at least three independent experiments. Comparisons were performed using Student's t-test and one-way ANOVA. Fer-1: ferrostatin-1; ns, not significant. ^∗∗∗p < 0.001 and ^###p < 0.001.