FIG. 2.

Purification and comigration of Dld and NoxA2 enzyme activities. Lane 1, partially purified sample from A. fulgidus obtained after phenyl-Sepharose fractionation (3.3 μg) was separated by SDS–10% PAGE and stained with Coomassie dye. Lane 2, the same phenyl-Sepharose fraction was incubated in Tris (pH 7.5) and then assayed with Tris, MgSO₄, PMS, MTT, and D-lactate to detect Dld activity and with NaPi, menadione, NBT, and NADH to detect NoxA2 activity.