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Reconstitution of active NADH oxidase after extraction of cofactor. (A) rNoxA2 (40 μg) and (B) NoxA2 (13 μg, total protein) were electrophoresed under denaturing conditions, renatured, and then assayed in the presence of cofactors for Nox activity. Activity was observed by the formation of a blue formazan precipitate from MTT. Lanes: 1, benchmark prestained protein standards; 2, no cofactor; 3, FAD; 4, F420; 5, FMN; 6, riboflavin.
