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. 2022 Oct 10;221(12):e202107134. doi: 10.1083/jcb.202107134

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© 2022 Weier et al.

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Figure S3. — Antigen encounter modifies the cell division cycle in DCs. (a) Time-lapse live-cell epifluorescence microscopy of unstimulated immature WT BMDCs progressing though mitosis. Merged images of DIC (gray) and Hoechst (blue) are shown. Frames were collected every 15 s. Only every second frame is shown in montage. See also Video 2 and Fig. 5 a. (b) Time-lapse live-cell epifluorescence microscopy of WT BMDCs stimulated with LPS. Merged images of DIC (gray) and Hoechst (blue) are shown. Frames were collected every 15 s. Only every second frame is shown in montage. See also Video 3 and Fig. 5 c. (c) Time-lapse live-cell epifluorescence microscopy of WT BMDCs stimulated with LPS showing impaired cleavage furrow ingression. Merged images of DIC (gray) and Hoechst (blue) are shown. White arrow indicates beginning of cleavage furrow ingression. White arrowheads denote regression of the cleavage furrow. Frames were collected every 15 s. Only every fourth frame is shown in montage. See also Video 4 and Fig. 5 e. All scale bars, 10 μm. Key images from this time lapse are highlighted in Fig. 5.