TABLE 3.
β-Galactosidase activity in P. aeruginosa PAO25 cells expressing the chiC′-lacZ or lasB′-lacZ fusion
| Plasmid | Fusion | β-Galactosidase activity (Miller units)a
|
|||||
|---|---|---|---|---|---|---|---|
| PAO25 (wild-type)
|
PAN32 (lasIR vsmI), LB | ||||||
| LB | 1/10 LB | 1/10 LB + glycerol | 1/10 LB + GlcNAc | 1/10 LB + chitin | |||
| pMP220 | 203 ± 28 | 225 | 63 | 58 | 44 | 169 ± 72 | |
| pJA5 | chiC′-lacZ | 7,758 ± 960 | 1,590 | 1,259 | 1,119 | 1,044 | 152 ± 42 |
| pPB107 | lasB′-lacZ | 12,383 ± 2,570 | ND | ND | ND | ND | 104 ± 51 |
a
When standard deviations are given, the results are from at least four independent measurements. Otherwise, the results of a representative experiment are shown. ND, not determined. Cells of PAO25 and PAN32 were grown overnight in LB or in 10-fold-diluted LB (1/10 LB) supplemented with a carbon source.