Table 1.

The information of methylation and qRT-PCR primers

Genes	Accession number	Sequence 5′ → 3′	Start	Tm (°C)	Product size (bp)	No. CpG
Methylation primers
NIS	NG_012930.1	F:GTGATTAGGGGATTATAGTGTATGG	− 775	57.13	210	7
		R:TAAATTACAAATTTATTAAACTCCC	− 988	52.69
qRT-PCR primers
NIS	NM_000453.3	F:CCCAGACCAGTACATGCCTC	–	59.82	86	–
		R:TGTAAGCACAGGCCAGGAA		58.85
Β-actin	NM_001101.5	F:GATCAAGATCATTGCTCCTCCT	–	57.65	108	–
		R:TACTCCTGCTTGCTGATCCA		58.43

Tm temperature