Table 2.
Sequences of the oligonucleotide primers used for quantitative real-time PCR for immune-related genes expreesiona
| Genes | Forward primer sequence (5′ → 3′) | Reverse primer sequence (5′ → 3′) | GenBank accession No | PCR size, bp |
|---|---|---|---|---|
| TLR4 | F:CCACTATTCGGTTGGTGGAC | R:ACAGCTTCTCAGCAGGCAAT | NM_001030693.1 | 120 |
| MyD88 | F:TGCAAGACCATGAAGAACGA | R:TCACGGCAGCAAGAGAGATT | NM_001030962.3 | 123 |
| NF-kB | F:TGGAGAAGGCTATGCAGCTT | R:CATCCTGGACAGCAGTGAGA | NM_205134.1 | 117 |
| IL-1β | F:TCATCTTCTACCGCCTGGAC | R:GTAGGTGGCGATGTTGACCT | NM_204524.1 | 149 |
| IL-6 | F:GATCCGGCAGATGGTGATAA | R:AGGATGAGGTGCATGGTGAT | NM_204628.1 | 126 |
| IL-8 | F-GGCTTGCTAGGGGAAATGA | R-AGCTGACTCTGACTAGGAAACTGT | NM_205498.1 | 200 |
| TNF-SF15 | F-CCCCTACCCTGTCCCACAA | R-TGAGTACTGCGGAGGGTTCAT | NM_204267.1 | 67 |
| IFN-γ | F:CTTCCTGATGGCGTGAAGA | R:GAGGATCCACCAGCTTCTGT | NM_205149.1 | 127 |
| TGF-β4 | F:AGAGCATTGCCAAGAAGCAC | R:GCAGTAGTCGGTGTCGAGGT | NM_001318456.1 | 119 |
| A20 | F:GAGAACGCAGAGCCTACACC | R:CCAACCTTCTTCCTGCACAT | NM_001277522.1 | 95 |
| Tollip | F:CATGGTACCTGTGGCAATACC | R:GCACTGAGCGGATTACTTCC | NM_001006471 | 122 |
| PI3K | F:AACATCTGGCAAAACCAAGG | R:CTGCAATGCTCCCTTTAAGC | NM001004410 | 150 |
| PIgR | F:ATGAAGCAGAGCCAGGAGAC | R:GAGTAGGCGAGGTCAGCATC | NM001044644.1 | 128 |
| MLCK | F:TTGACATGGAGGTTGTGGAA | R:GAAGTGACGGGACTCCTTGA | NM_001322361.1 | 119 |
| Claudin1 | F:AAGTGCATGGAGGATGACCA | R:GCCACTCTGTTGCCATACCA | NM_001013611.2 | 119 |
| Occludin | F:AGTTCGACACCGACCTGAAG | R:TCCTGGTATTGAGGGCTGTC | NM_205128.1 | 124 |
| ZO-1 | F:ACAGCTCATCACAGCCTCCT | R:TGAAGGGCTTACAGGAATGG | XM_015278981.1 | 125 |
| ZO-2 | F:CACCACCACCTGTTTCTGTG | R:TTCACTCCCTTCCTCTTCCA | NM_204918.1 | 119 |
aPrimers were designed and synthesized by Sango Biotech (Shanghai, China) Co., Ltd. F: forward, R: reverse
TLR Toll-like receptor, MyD88 myeloid differential protein-88, TRAF-6 TNF receptor-associated factor 6, NF-κB nuclear factor kappa-light-chain-enhancer of activated B cells, TNFSF15 tumor necrosis factor superfamily member 15, IL interleukin, IFN-γ interferon γ, Tollip Toll-interacting protein, PI3K phosphatidylinositol 3-kinase, A20 protein A20, SOCS suppressor of cytokine signaling, ZO-1 zonula occludens-1, EGFR epidermal growth factor receptor, GLP-2 lucagon-like peptide-2, IGF-2 insulin-like growth factor-2, TGF- β3 transforming growth factor beta 3