FIG 2.

Liposomes effectively bind HA trimers to the membrane surface. (A) Competitive binding assay with his-tag binding CoPoP/PHAD liposomes and Nickel-NTA beads. Liposomes incubated in 1:1, 1:2, and 1:4 ratios of HA:CoPoP (HA antigen from A/Ca/Illinois/11613/2015), with nonbinding PoP/PHAD liposomes for negative control. Sup., supernatant fraction; Beads, bead fraction. (B) Average liposome size. (C) Polydispersity of liposomes. (D) TEM images of CoPoP liposomes. (E) CoPoP liposomes bound with influenza virus HA antigen at 2:1 mass ratio. Error bars in panels B and C indicate standard deviation.