FIG 6.

Production and quality control of flavivirus NS1 site-directed mutants. Proteins were evaluated by silver stain following SDS-PAGE gel (A) and native PAGE (B, C). Two micrograms of each NS1 construct were used. For native gels, proteins were detected using anti-His MAb. Top-row labels indicate the flavivirus NS1 backbone. Numbering under bands refers to the residues that were swapped between DENV and WNV NS1. (A) Silver stain of site-directed NS1 mutants between DENV and WNV NS1. (B, C) Native PAGE of site-directed NS1 mutants between DENV and WNV NS1, divided into two gels each with respective WT DENV and WNV NS1 controls. WT, wild type.