FIG 3.

One-step growth kinetics of MuV in Treacle-knockdown cells. A549 cells were transfected with siNC or siTreacle. At 72 h posttransfection, the cells were infected with rMuV-AcGFP at a multiplicity of infection (MOI) of 3 and then cultured for 0, 8, 16, or 24 h. (A, B) The expression levels of genomic RNA (A) and mRNA (B) were determined by reverse transcription-quantitative PCR (qRT-PCR). The values were normalized to the value for the control gene HPRT1. (C) The expression of N protein was evaluated by Western blotting. (D) The viral titer in the supernatant was determined by a plaque assay. The presented data are the means of triplicate measurements, with error bars indicating the SD. The significance of the difference between siNC-treated and siTreacle-treated cells was determined by a Student’s t test. *, P < 0.05; ns, not significant.