Fig. 2.

CircEZH2 accentuate vitality, migration, and invasion of BC cells in vitro. a Here is a diagram of mechanisms about circEZH2 overexpression with plasmid and knockdown with siRNA. b We determined the success of knockdown as well as overexpression of circEZH2 using RT-qPCR. c-e To evaluate the vitality in BC cells, EdU assays (Scale bar, 50 μm), colony formation assays and CCK8 assays were conducted. f and g Migration, invasion of transwell (Scale bar 100 μm) and wound-healing assays (Scale bar 200 μm) were conducted to determine the ability of metastasis in BC cells. The data was revealed as the mean ± SD and all experiments were repeated at least three times, * p < 0.05; ** p < 0.01; *** p < 0.001