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. 2022 Oct 10;17:4725–4741. doi: 10.2147/IJN.S381170

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© 2022 Chen et al.

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Cytotoxicity and apoptosis assays of M1/IR780@PLGA nanoparticles. Cell viabilities of HUVEC (A) and 4T1 cells (B) after various treatments. Data are expressed as means SD (n = 3). Statistical significances were calculated via Student’s t-test, *P < 0.05. (C) Relative quantitative and (D) CLSM images analysis of Calcein AM/PI co-stained 4T1 cells after various treatments (live and dead cells are stained green and red, respectively). Data are expressed as means SD (n = 3). Statistical significances were calculated via one-way ANOVA, ***P < 0.001, ns means no statistical significance.