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. Author manuscript; available in PMC: 2022 Oct 12.
Published in final edited form as: Lab Chip. 2019 Jan 15;19(2):306–315. doi: 10.1039/c8lc00825f

Fig. 5: Effects of local hypoxia on cells in a microfluidic co-culture device.

Fig. 5:

Hypoxia was induced in the left culture chamber for 2 h, then cells in both chambers were stained with the hypoxic probe. a) Staining for actin (phalloidin -green) and nuclei (Dapi - blue) demonstrating similar cell distribution, density and morphology in both culture chambers. b) Immunofluorescent staining for PMDZ adducts demonstrate that cells in the left chamber were exposed to hypoxic conditions (pO2 < 10mmHg) while cells in the right chamber remained normoxic. c) Immunofluorescent staining for PMDZ(red), actin (phalloidin -green) and nuclei (Dapi - blue) to show a circular hypoxic pattern. The scale bars are 200μm in all figures.