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Sodium dithionite-reduced minus potassium ferricyanide-oxidized difference spectra on wild-type and cyd mutant membranes. (A) mc2155, (B) mc2155(pOTBCYD), (C) cydA::aph, (D) cydA::aph(pOTBCYD), (E) cydB::aph, or (F) cydB::aph(pOTBCYD). In all cases, samples were suspended in 1 ml of TC buffer (pH 7.4) at a protein concentration of 2.6 mg/ml.
