FIG. 2.

RH protein stability in the hyp deletion strains. R. eutropha strains harboring plasmid pGE378 for RH overproduction were grown in FGN medium under hydrogenase derepressing conditions. The presence of the RH large subunit HoxC in soluble extracts was analyzed by the immunoblot technique. A total of 20 μg of protein was applied to each lane. Lane 1, HF470; lane 2, HF510 (containing control vector pEDY309 instead of pGE378); lane 3, HF503; lane 4, HF504; lane 5, HF505; lane 6, HF506; lane 7, HF507; lane 8, HF508; lane 9, HF509.