TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| R. eutropha | ||
| H16 | Wild type, SH+ MBH+ RH+ HoxJ− (hoxJg1264a) | DSM428, ATCC 17699 |
| HF470 | SH− (hoxHΔ) MBH− (hoxGΔ) RH+ | This study |
| HF510 | SH− (hoxHΔ) MBH− (hoxGΔ) RH− (hoxCΔ) | This study |
| HF410 | hypA1Δ hypA2Δ HoxJ− | 45 |
| HF417 | hypB1Δ hypB2Δ HoxJ− | 45 |
| HF441 | hypF1Δ hypF2Δ HoxJ− | 45 |
| HF340 | hypCΔ HoxJ− | 10 |
| HF338 | hypDΔ HoxJ− | 10 |
| HF339 | hypEΔ HoxJ− | 10 |
| HF469 | hypXΔ HoxJ− | 6 |
| HF503 | hypA1Δ hypA2Δ SH− (hoxHΔ) MBH− (hoxGΔ) | This study |
| HF504 | hypB1Δ hypB2Δ SH− (hoxHΔ) MBH− (hoxGΔ) | This study |
| HF505 | hypF1Δ hypF2Δ SH− (hoxHΔ) MBH− (hoxGΔ) | This study |
| HF506 | hypCΔ SH− (hoxHΔ) MBH− (hoxGΔ) | This study |
| HF507 | hypDΔ SH− (hoxHΔ) MBH− (hoxGΔ) | This study |
| HF508 | hypEΔ SH− (hoxHΔ) MBH− (hoxGΔ) | This study |
| HF509 | hypXΔ SH− (hoxHΔ) MBH− (hoxGΔ) | This study |
| HF573 | SH− (hoxHΔ) MBH− (hoxGΔ) nor(R2A2B2)Δ::Φ(hoxK′-lacZ) | This study |
| HF439 | hyp1Δ (hyp[A1B1F1CDEX]Δ) | This study |
| HF575 | hyp1Δ hyp2Δ (hyp[A1B1F1CDEX]Δ) (hyp[A2B2F2]Δ) | This study |
| HF581 | hyp1Δ hyp2Δ SH− (hoxHΔ) MBH− (hoxGΔ) RH+nor(R2A2B2)Δ::Φ(hoxK′-lacZ) | This study |
| HF582 | hyp1Δ hyp2Δ SH− (hoxHΔ) MBH− (hoxGΔ) RH− (hoxCΔ) nor(R2A2B2)Δ::Φ(hoxK′-lacZ) | This study |
| E. coli | ||
| JM109 | F′ traD36 lacIq Δ(lacZ)M15 proA+B+/e14− (McrA−) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17(rK− mK+) relA1 supE44 recA1 | 46 |
| S17-1 | Tra+recA pro thi hsdR chr::RP4-2 | 39 |
| Plasmids | ||
| pBluescript SK− | Apr, lacZ′, T7 gene 10 promoter, f1 ori | Stratagene Cloning Systems |
| LITMUS 28 | Apr, lacZ′, ColE1 ori | New England Biolabs |
| pLO1/pLO2 | Kmr, sacB, RP4 oriT, ColE1 ori | 22 |
| pEDY309 | RK2 ori, Tcr, Mob+, promoterless lacZ gene | 18 |
| pCH297 | 3.5-kb KpnI fragment containing ′hypDEXhoxA′ in pBluescript SK− | 22 |
| pCH412 | 2.1-kb SmaI-PvuII fragment containing hypA1Δ in pLO1 (PmeI cut) | 9 |
| pCH547 | 2.7-kb fragment containing a 6.3-kb in-frame deletion in hypA1B1F1CDEX in pLO1 | This study |
| pCH371 | 2.7-kb KpnI-XbaI fragment containing hypB1F1 in pTZ18R | J. Dernedde and B. Friedrich |
| pCH424 | pLO1 with a 2.2-kb SalI-SmaI fragment containing hoxGΔ | 4 |
| pCH455 | 15-kb HindIII fragment of pGE15 containing the SH gene region in pBluescript KS+ | 27 |
| pCH474 | pLO1 with a 976-bp SacI fragment containing hoxHΔ | 27 |
| pCH615 | 0.8-kb Eco47III fragment containing ′hoxJ into PmeI-digested pLO3 | 21 |
| pCH644 | pLO1 with a 2.45-kb PstI fragment containing hoxCΔ | 21 |
| pCH857 | 5.1-kb Ecl136II-BglII fragment of pCH455 cloned into LITMUS 28 (EcoRV-BglII cut) | This study |
| pCH858 | 4.1-kb fragment of a PvuII partial digest of pCH857, religated | This study |
| pCH859 | 1.4-kb BglII fragment (Klenow treated) of pCH858 cloned into the PmeI site of pLO2 | This study |
| pCH872 | nor(R2A2B2)Δ::Φ(hoxK′-lacZ) in pLO1 | O. Lenz and B. Friedrich |
| pGE6 | 8.6-kb EcoRI fragment containing the hyp1 region in pVK101 | 12 |
| pGE15 | 15.0-kb HindIII fragment of pHG1 in pVK101 | 42 |
| pGE151 | Derivative of pRK404 | 19 |
| pGE301 | Φ(hoxK′-lacZ), Tcr | 22 |
| pGE378 | pEDY309 with a 2.8-kb HindIII-XbaI fragment containing PSH-hoxB-hoxC and with a 2.2-kb PvuII-Ecl136II fragment containing Plac-hoxA | 18 |
| pGE457 | 1.4-kb PstI-EcoRV fragment of pCH371 containing hypF1 cloned into PstI-Ecl136II-cut pGE151 | This study |
a
Nalr, nalidixic acid resistant; Tcr, tetracycline resistant; Apr, ampicillin resistant; Kmr, kanamycin resistant.