Fig 1. Experimental design of the in vitro interaction model between F. hepatica juveniles (FhNEJ) and mouse intestinal epithelial cells (MPSIEC).
(A) Parasitic extracts and host cell lysates were obtained before (FhNEJ 1, MPSIEC 1) and after co-culturing for 3 h (FhNEJ 2, MPSIEC 2) and their proteins analyzed by SWATH-MS. (B, C, D) Representative images of MPSIEC (at the background of each image) stimulated with the FhNEJ (arrows) are shown (optical microscope, 10x). Panel A created with BioRender.com.
