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. 2022 Oct 12;12:17126. doi: 10.1038/s41598-022-21587-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sex difference in relative miR-223-3p expression in F4/80+ and Ly6G+ cells at 24 h post GBS lung infection. F4/80+ cells and Ly6G+ cells were purified from the lungs of GBS-infected or naive male and female mice (n = 6/group) using F4/80 and Ly6G immunomagnetic antibodies. Comparative analysis of miR-223-3p expression in the F4/80+ (A) and in the Ly6G+ (B) cell populations was evaluated by real-time qPCR. The values were normalized with two internal controls, snoRNA-202 and snoRNA-234. *p < 0.05 (Mann–Whitney U test). Data are representative of two independent experiments. The percentage of CD80+ and CD206+ macrophages were evaluated by flow cytometry with the gating strategy shown in Fig. S4. Correlation between the miR-223-3p expressed by the F4/80+ cells and the percentage of M1 or M2 macrophages (C). *p < 0.05, ****p < 0.0001 (Pearson χ² test).